other:Article Title: Bacterial infection disrupts established germinal center reactions through monocyte recruitment and impaired metabolic adaptation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Human/Mouse CD45R/B220 -176Yb antibody (clone RA3-6B2) Fluidigm Cat# 3176002B, RRID:AB_2895123 Anti-Mouse CD170 (Siglec-F) -163Dy antibody (clone S17007L) BioLegend, conjugated in house Cat# 155502, RRID:AB_2810420 Anti-Mouse CD95 (Fas) -166Er antibody (clone SA362F3) BioLegend, conjugated in house Cat# 152602, RRID:AB_2629777 Anti-Mouse CD185 (CXCR5) -154Sm antibody (clone L138D7) BioLegend, conjugated in house Cat# 145502, RRID:AB_2561955 Anti-Mouse CD138 (Syndecan-1) -162Dy antibody (clone 281-2) BioLegend, conjugated in house Cat# 142502, RRID:AB_10965646 Anti-Mouse Ly-6A/E (Sca-1) -147Sm antibody (clone D7) BioLegend, conjugated in house Cat# 108102, RRID:AB_313339 Rabbit anti-mouse MCT4 antibody (clone H-90) Santa Cruz Biotechnology Cat# sc-50329, RRID:AB_2189333 FITC anti-MU/HU GL7 Antigen antibody (clone GL7) BioLegend Cat# 144603, RRID:AB_2561696 Alexa Fluor(R) 647 anti-mouse IgD antibody (clone 11-26c.2a) BioLegend Cat# 405708, RRID:AB_893528 aCCR2 mAb (clone MC21) Prof. Steffen Jung N/A InVivoMab anti-mouse Ly6G antibody (clone 1A8) Bio X Cell Cat# BE0075-1, RRID:AB_1107721 InVivoMab anti-mouse TNFa antibody (clone XT3.11) Bio X Cell Cat# BE0058, RRID:AB_1107764 Ultra-LEAF(TM) Purified anti-mouse IFN-gamma antibody (clone R4-6A2) BioLegend Cat# 505711, RRID:AB_2832807 InVivoMab anti-mouse NK1.1 antibody (clone PK136) Bio X Cell Cat# BE0036, RRID:AB_1107737 InVivoPlus mouse IgG2b isotype control antibody (clone MPC11) Bio X Cell Cat# BP0086, RRID:AB_2894741 Bacterial and virus strains Bacillus subtilis (strain 3610) Prof. Eran Elinav N/A Listeria monocytogenes (strain 10403) Dr. Roi Avraham N/A Salmonella typhimurium (strain SL1344) Prof. Eran Elinav N/A Plasmodium yoelii Prof. Neta-Regev Rudzki N/A GFP- Salmonella typhimurium (strain SL1344) Dr. Roi Avraham N/A Recombinant influenza X31-GP33 virus Dr. Michelle Linterman N/A Staphylococcus aureus (strain ATCC25923) Prof. Eran Elinav N/A Chemicals, peptides, and recombinant proteins LightCycler 480 SYBR Green I Master Roche Cat# 04707516001 Tissue-Tek O.C.T.
Article Title: Bacterial infection disrupts established germinal center reactions through monocyte recruitment and impaired metabolic adaptation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Mouse CD45-89Y antibody (clone 30-F11) Fluidigm Cat# 3089005B, RRID:AB_2651152 Anti-Mouse I-A/I-E -209Bi antibody (clone M5/114.15.2) Fluidigm Cat# 3209006B, RRID:AB_2885025 Anti-Mouse Ly-6G -141Pr antibody (clone 1A8) Fluidigm Cat# 3141008B, RRID:AB_2814678 Anti-Mouse CD11c -142Nd antibody (clone N418) Fluidigm Cat# 3142003B, RRID:AB_2814737 Anti-Mouse CD69 -145Nd antibody (clone H1.2F3) Fluidigm Cat# 3145005B, RRID:AB_2895115 Anti-Mouse CD115 -144Nd antibody (clone AFS98) Fluidigm Cat# 3144012B, RRID:AB_2895116 Anti-Mouse CD14 -143Nd antibody (clone Sa14-2) BioLegend, conjugated in house Cat# 123321, RRID:AB_2563720 Anti-Mouse F4/80 -146Nd antibody (clone BM8) Fluidigm Cat# 3144012B, RRID:AB_2895117 Anti-Mouse CD11b -148Nd antibody (clone M1/70) Fluidigm Cat# 3148003B, RRID:AB_2814738 Anti-Mouse Ly-6C -150Nd antibody (clone HK1.4) Fluidigm Cat# 3150010B, RRID:AB_2895118 Anti-Mouse CD64 -151Eu antibody (clone X54-5/7.1) Fluidigm Cat# 3151012B, RRID:AB_2814680 Anti-Mouse CD3e -152Sm Antibody (clone 145-2C11) Fluidigm Cat# 3152004, RRID:AB_2687836 Anti-Mouse CD16/32 -153Eu antibody (clone 93) Fluidigm Cat# 3153011B, RRID:AB_2895119 Anti-Mouse MERTK -155Gd antibody (clone 2B10C42) BioLegend, conjugated in house Cat# 151502, RRID:AB_2566624 Anti-Mouse CD196(CCR6) -156Gd antibody (clone 29-2L17) Fluidigm Cat# 3156016B, RRID:AB_2895120 Anti-Mouse CD169 -174Yb antibody (clone 3D6.112) BioLegend, conjugated in house Cat# 142402, RRID:AB_10916523 Anti-Mouse CD279/PD-1 -159Tb Antibody (clone 29F.1A12) Fluidigm Cat# 3159024, RRID:AB_2687839 Anti-Mouse CD80 -160Gd antibody (clone 16-10A1) Fluidigm Cat# 3171008B, RRID:AB_2885024 Anti-Mouse/Rat CD40 -161Dy antibody (clone HM40-3) Fluidigm Cat# 3161020B, RRID:AB_2885020 Anti-Mouse CX3CR1 -164Dy antibody (clone SA011F11) Fluidigm Cat# 3164023B, RRID:AB_2832247 Anti-Mouse CD103 -167Er antibody (clone 2E7) BioLegend, conjugated in house Cat# 121402, RRID:AB_535945 Anti-Mouse CD8a -168Er antibody (clone 53-6.7) Fluidigm Cat# 3168003B, RRID:AB_2811241 Anti-Human/Mouse CD44 -171Yb antibody (clone IM7) Fluidigm Cat# 3171003B, RRID:AB_2895121 Anti-Mouse CD38 -175Lu antibody (clone 90) Fluidigm Cat# 3175014B, RRID:AB_2895122 Anti-Mouse CD19 -149Sm antibody (clone 6D5) Fluidigm Cat# 3149002B, RRID:AB_2814679 Anti-Mouse CD62L -173Yb antibody (clone MEL-14) BioLegend, conjugated in house Cat# 104443, RRID:AB_2562802 Anti-Mouse CD4 -172Yb antibody (clone RM4-5) Fluidigm Cat# 3172003B, RRID:AB_2811242 (Continued on next page) e2 Immunity 55, 442–458.e1–e8, March 8, 2022
|